Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6129363 | Clinical Microbiology and Infection | 2015 | 16 Pages |
Abstract
Molecular methods are crucial for mucormycosis diagnosis because cultures are frequently negative, even if microscopy suggests the presence of hyphae in tissues. We assessed PCR/electrospray-ionization mass spectrometry (PCR/ESI-MS) for Mucorales identification in 19 unfixed tissue samples from 13 patients with proven or probable mucormycosis and compared the results with culture, quantitative real-time PCR, 16S-23S rRNA gene internal transcribed spacer region (ITS PCR) and 18S PCR sequencing. Concordance with culture identification to both genus and species levels was higher for PCR/ESI-MS than for the other techniques. Thus, PCR/ESI-MS is suitable for Mucorales identification, within 6 hours, for tissue samples for which microscopy results suggest the presence of hyphae.
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Authors
A. Alanio, D. Garcia-Hermoso, S. Mercier-Delarue, F. Lanternier, M. Gits-Muselli, J. Menotti, B. Denis, A. Bergeron, M. Legrand, O. Lortholary, S. Bretagne, French Mycosis Study Group French Mycosis Study Group,