| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6131836 | Current Opinion in Microbiology | 2015 | 6 Pages |
Abstract
In this review we briefly describe the development of CRISPR tools for genome editing and control of transcription in bacteria. We focus on the Type II CRISPR/Cas9 system, provide specific examples for use of the system, and highlight the advantages and disadvantages of CRISPR versus other techniques. We suggest potential strategies for combining CRISPR tools with high-throughput approaches to elucidate gene function in bacteria.
Related Topics
Life Sciences
Immunology and Microbiology
Microbiology
Authors
Jason M Peters, Melanie R Silvis, Dehua Zhao, John S Hawkins, Carol A Gross, Lei S Qi,