Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6132564 | Current Opinion in Microbiology | 2009 | 7 Pages |
Abstract
Genome-scale screens for intraviral and virus-host protein interactions and the analysis of literature-curated datasets are able to provide a novel, comprehensive perspective of viruses, and virus-infected cells. Until now, large-scale interaction screens were predominantly performed with the yeast-two-hybrid (Y2H) system; however, alternative high-throughput technologies detecting binary protein interactions or protein complexes have been developed. Although many of the previous studies suffer from a rather poor validation of the results and few biological implications, these technologies potentially lead to a plethora of novel hypotheses. Here, we will give an overview of current approaches and their technical limitations, present recent examples and novel developments.
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Authors
SM Bailer, J Haas,