Variation of saturated surface density of ovalbumin on bubble surface in continuous foam separation

The adsorption of ovalbumin (OA) onto the bubble surfaces was studied with various pHs (3.5, 4.6, 6.0 and 8.0) by a continuous foam separation technique. From the value of the saturated surface density of adsorbed OA, the variation of effective diameter (D) of an OA molecule on the bubble surface was estimated for various pHs (3.5, 4.6, 6.0 and 8.0) of the OA solutions, assuming that the cross section of the OA molecules be circular and that the OA molecules adsorb on the bubble surface in a closest packing structure. The estimated variation of D with pH was attempted to explain based on a model modified from that proposed by Pujar and Zydney [N.S. Pujar, A.L. Zydney, J. Chromatogr. A 796 (1998) 229–238]. The modified model could well reproduce the variation of the effective diameter with pH; the values of D calculated on the basis of the modified model almost agreed with that estimated from the saturated surface density in the present experimental pH range. From these, conclusion was drawn that the modified model presented in this study can express the variation in the effective diameter with pH.

Graphical abstractIn this work, variation of sphere-equivalent diameter of ovalbumin adsorbed on bubble surface in saturated state with pH was attempted to explain based a model modified from that proposed by Pujar and Zydney (1998).Figure optionsDownload full-size imageDownload as PowerPoint slide