Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6136253 | Microbial Pathogenesis | 2014 | 6 Pages |
Abstract
“Nanobacteria”, also known as nanons or calciprotein particles (CPP), are nano-sized protein mineral complexes which have been isolated from numerous biological sources. Nanons possess self-replication properties and contain only serum proteins (e.g. Fetuin-A, Albumin). Herein, we develop a simplified in vitro model of nanons propagation composed of only fetuin-A as a protein. Using this model, we demonstrate that fetuin from nanons possesses a different, non-native conformation. Moreover, we show that nanons induce soluble fetuin-A precipitation which could serve as a template for calcification. This phenomenon explains the observed self-propagating properties that mimic infectious behavior. We also demonstrate that renal calculi are capable of inducing a conformational change in fetuin-A, suggesting that the propagation phenomenon of nanons may occur in vivo.
Keywords
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Authors
Eric Chabrière, Daniel Gonzalez, Said Azza, Pierrick Durand, Farooq A. Shiekh, Valerie Moal, Jean-Pierre Baudoin, Isabelle Pagnier, Didier Raoult,