Different NF-κB activation characteristics of human respiratory syncytial virus subgroups A and B

► We compare NF-κB activation by respiratory syncytial viruses from A and B subgroups. ► Purified viruses infected both continuous and primary cell cultures. ► NF-κB activation measured by quantitative and qualitative approaches. ► All analyses show subgroup A induces increased NF-κB activation compared to subgroup B.