| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6196898 | Experimental Eye Research | 2014 | 12 Pages |
Abstract
â¢We present a detailed 3-step protocol for live imaging of polarized primary RPE.â¢Step 1: a protocol for primary porcine retinal pigment epithelial culture.â¢This method results in highly polarized monolayers within 7 days.â¢Step 2: two gene delivery protocols that yield efficient transgene expression.â¢Step 3: High speed live imaging to visualize intracellular traffic in the RPE.
Related Topics
Life Sciences
Immunology and Microbiology
Immunology and Microbiology (General)
Authors
Kimberly A. Toops, Li Xuan Tan, Aparna Lakkaraju,