Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6196898 | Experimental Eye Research | 2014 | 12 Pages |
Abstract
â¢We present a detailed 3-step protocol for live imaging of polarized primary RPE.â¢Step 1: a protocol for primary porcine retinal pigment epithelial culture.â¢This method results in highly polarized monolayers within 7 days.â¢Step 2: two gene delivery protocols that yield efficient transgene expression.â¢Step 3: High speed live imaging to visualize intracellular traffic in the RPE.
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Authors
Kimberly A. Toops, Li Xuan Tan, Aparna Lakkaraju,