| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6238 | Biomaterials | 2014 | 10 Pages |
Abstract
Cationic polymer PEI-CyD is doped with Nd by plasma technology to produce the gene vector: Nd@PEI-CyD. Luciferase expression and EGFP transfection experiments performed in vitro reveal that Nd@PEI-CyD has significantly higher transfection efficiency than lipofectamine 2000 and PEI-CyD and the mechanism is studied and proposed. The rare-earth element, Nd, stimulates the energy metabolism of cells, enhances cell uptake of complexes/pDNA, and regulates the cellular pathways. These special features suggest a new strategy involving metal-incorporated non-viral gene vectors.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Qiwen Wang, Weihong Jin, Guosong Wu, Ying Zhao, Xue Jin, Xiurong Hu, Jun Zhou, Guping Tang, Paul K. Chu,