Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6450941 | Biomaterials | 2017 | 9 Pages |
Abstract
We introduce a versatile carrier system for in vitro and in vivo immune stimulation based on soft matter DNA nanoparticles (NPs). The incorporation of lipid-modified nucleotides into DNA strands enables the formation of micelles of uniform size. In a single self-assembly step, the micelles can be equipped with immune adjuvant (CpG) motifs and fluorescent probes. The immunological effects of CpG confined at the NP surface were studied in a comprehensive manner in animal experiments. Dose-dependent activation of spleen dendritic cells (DCs) by CpG-conjugated NP was observed, which was accompanied by the pronounced up-regulation of co-stimulatory molecule and cytokine production.
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Authors
Jun-O Jin, Haein Park, Wei Zhang, Jan Willem de Vries, Agnieszka Gruszka, Myung Won Lee, Dae-Ro Ahn, Andreas Herrmann, Minseok Kwak,