| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6451448 | Current Opinion in Biotechnology | 2017 | 7 Pages |
•CRISPR/Cas9 enables system-level genome and metabolic engineering.•Expression-tuning of metabolic pathways can be achieved with dCas9.•CRISPR/Cas9-mediated genome-wide perturbations and sequencing allows mapping of phenotypes onto genotypes.
CRISPR/Cas9 (clustered regularly interspaced palindromic repeats and the associated protein Cas9) techniques have made genome engineering and transcriptional reprogramming studies more advanced and cost-effective. For metabolic engineering purposes, the CRISPR-based tools have been applied to single and multiplex pathway modifications and transcriptional regulations. The effectiveness of these tools allows researchers to implement genome-wide perturbations, test model-guided genome editing strategies, and perform transcriptional reprogramming perturbations in a more advanced manner than previously possible. In this mini-review we highlight recent studies adopting CRISPR/Cas9 for systems-level perturbations and model-guided metabolic engineering.
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