Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6451517 | Current Opinion in Biotechnology | 2018 | 7 Pages |
â¢Proteins with a SPX domain are involved in phosphate homeostasis in eukaryotes.â¢SPX domain binds inositol pyrophosphates at high affinity.â¢Inositol pyrophosphates promote the interaction of SPX with other proteins.â¢Inositol pyrophosphates are a key component of phosphate-deficiency signaling.
Proteins containing a SPX domain are involved in phosphate (Pi) homeostasis, including Pi transport and adaptation to Pi deficiency. The SPX domain harbors a basic surface binding Pi at low affinity and inositol pyrophosphates (PP-InsPs) at high affinity. Genetic and biochemical studies revealed that PP-InsPs serve as ligands for the SPX domain. Residues in the PHO1 SPX domain involved in PP-InsPs binding are critical for its Pi export activity, and the interaction between SPX proteins and the PHR1 transcription factor, which results in PHR1 inactivation, is promoted by PP-InsPs. Changes in PP-InsPs levels in response to Pi deficiency may thus contribute to the adaptation of plants to stress via the modulation of the activity of SPX-containing proteins and their interactors. Modulating PP-InsP levels or the affinity/specificity of the SPX domain for PP-InsP could potentially be used to engineer crops to maintain high yield under reduced Pi fertilizer input.
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