Article ID Journal Published Year Pages File Type
6451957 Journal of Biotechnology 2017 9 Pages PDF
Abstract

•Lipase-catalyzed transesterification of sucrose and vinyl caprate was carried-out.•Vinyl caprate was dispersed in a concentrated solution of sucrose and lipase in DMSO.•Lipase pretreatment greatly improved catalytic activity and reaction productivity.•2-O-acylated sucrose monoester was obtained as the major isomer in the final product.

Sucrose monocaprate was synthesized by carrying out a lipase-catalyzed transesterification in a non-aqueous biphasic medium. Vinyl caprate was mechanically dispersed into a solution of sucrose in DMSO. The use of DMSO allowed increasing sucrose concentration up to 0.7 M (in DMSO). The denaturing effect of DMSO on lipase was avoided by pretreatment of lipase by pH adjustment in the presence of crown ether. This pretreatment maintained a significant catalytic activity which led to 0.2 M sucrose monoester within 1 h at 50 °C, which represented higher productivity than already reported. Detailed structural characterization revealed that only monoester was recovered and the 2-O-acylated sucrose monocaprate was the major isomer in the final product.

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Physical Sciences and Engineering Chemical Engineering Bioengineering
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