Nucleotide incorporated magnetic microparticles for isolation of DNA

•A nucleotide incorporated polymeric microparticles were synthesized.•The use of a nucleotide as a ligand provide the interaction (ligand and DNA) natural and selective.•The polymeric material provide high purity of DNA in a short interaction time.•Very costly effective and a simple method was developed as compared to conventional techniques.•All corrections have been made.

For the comprehensive understanding of many diseases, especially genetic disease, the highly purified deoxyribonucleic acid (DNA) is needed to perform in-detail studies. There are various methods to obtain highly purified DNA, but low in number. In this study, Fe&Ni-mc-poly (2-hydroxyethyl methacrylate-adenine methacrylate) [poly(HEMA-AdeM)] microparticles were synthesized to get high purification ratio because of natural and thus selective interaction between adenine on the microparticles and thymine on the DNA molecule and also high amount of DNA purified. For the characterization of microparticles scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), elemental analysis, swelling ratio and zeta size analysis methods were performed. All experiments were performed batch-wise for the determination of optimum conditions such as pH, initial DNA concentration, temperature and interaction time. At the end of the all study, the desorption yield was obtained as 97.7% and after 5 adsorption-desorption cycle, the adsorption capacity was decreased only 3%.

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