Mediated spectroelectrochemical determination of holo-transferrin reduction potential using a flow cell with disposable screen-printed indium‑tin oxide electrode

•Simple handling, small volume, easy electrode replacement and automated measurement.•Quick setup with all materials and accessories commercially available.•Convenient for characterization of other redox-active biomolecules.

A convenient spectroelectrochemical method for the determination of holo-transferrin reduction potential is described. All materials and accessories are commercially available and the results are in agreement with previous reports that required custom-built hardware. The method requires minimal preparation with simple sample handling, small solution volume, cost-effective electrode replacement and automated measurement. This method can easily be applied to the study of similar systems in aqueous media and might be of particular importance in the momentous field of glycomics, where large numbers of protein isoforms need to be characterized.