An origami paper device for complete elimination of interferents in enzymatic electrochemical biosensors

•Eliminate electroactive interferents completely based on an origami paper device•Controlled successive electrochemical reactions of interferents and substrates•An interference-free and calibration-free coulometric glucose biosensor•A widely adaptable approach for interference-free electrochemical biosensors

Many efforts have been made to prevent interferences in enzymatic electrochemical biosensors by permselective membranes or mediators with low redox potential. However, it is difficult to completely eliminate interferents without compromised sensitivity by these traditional procedures. We propose here a method based on an origami paper device that separates the electrochemical reactions of interferents and substrates for complete depletion of interferents and precise analysis of substrates. Interferents such as ascorbate, urate and paracetamol were completely consumed by a simple electrolysis step, while substrates were quantitatively analyzed by coulometry. With GOx as a model enzyme, an interference-free and calibration-free coulometric glucose biosensor has been demonstrated successfully. The proposed origami paper device provides a facile and easy-controlled approach to eliminate the electroactive interferents completely for enzymatic electrochemical biosensors.

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