Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6482532 | Bioresource Technology Reports | 2018 | 24 Pages |
Abstract
The present work aimed to characterize and to improve the production of the siderophore produced by Kosakonia radicincitans and apply it to control of phytopathogenic fungi. The siderophore, characterized by liquid chromatography coupled to tandem mass detection (LC-MS/MS), was identified as enterochelin. The improvement of its production was carried out using different conditions. The higher production of enterochelin (45â¯mgâ¯gâ1 dry weight biomass) was reached in a medium with lactose as the carbon source, 0.82â¯Î¼molâ¯Lâ1 of iron, at 28â¯Â°C and 200â¯rpm. The siderophore at 60â¯mgâ¯Lâ1 of concentration inhibited the conidia germination and the mycelial growth of Penicillium expansum, Botrytis cinerea, Rhizopus sp., Alternaria sp. and Cladosporium cladosporioides. Biocontrol of Botrytis cinerea and Penicillium expansum were performed in apple fruit using Kosakonia radicincitans and enterochelin, alone or in a mixture. The mixture was the more effective. In this case, the reduction of decay provoked for B. cinerea reached 52%.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Yesica Lambrese, MarÃa Guiñez, Viviana Calvente, Gabriela Sansone, Soledad Cerutti, Julio Raba, MarÃa Isabel Sanz,