NF-κB decoy oligodeoxynucleotide mitigates wear particle-associated bone loss in the murine continuous infusion model

Upper panel, illustration of the murine model with continuous femoral infusion. Mouse distal femurs were exposed to ultra-high molecular weight polyethylene (UHMWPE) particles together with NF-κB decoy oligodeoxynucleotide (ODN) and appropriate controls. Lower panel, trabecular bone structure (blue square) in the distal femur was reconstructed into a 3D image. Yellow lines indicate the major bone loss area induced by UHMWPE particles. Green dotted circle indicated the inserted titanium rod channel from intercondylar region at distal femur. The number of infiltrated macrophages (Mac) and osteoclasts (OC) were determined by immunohistochemistry. UNT: Untreated control.112