Comparison of human olfactory and skeletal MSCs using osteogenic nanotopography to demonstrate bone-specific bioactivity of the surfaces

We have compared the ability of purified human mesenchymal stem cells isolated from the bone (hBM-MSC), antibody purified BM-MSC (BM271-MSCs), and antibody purified MSCs from the olfactory mucosa (hOM-MSC) to differentiated to bone and fat on nanotopographically embossed polycaprolactone (PCL). As seen for osteocalcin (above) MSCs from a neural cellular niche express similar bone induced cues to BM-MSCs suggesting that MSCs that inherently support nervous tissue can differentiate along the bone lineage in a similar manner to MSCs from a skeletal environment.150