Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6483898 | Biochemical Engineering Journal | 2018 | 37 Pages |
Abstract
A two-stage selection process was applied to eight oleaginous yeast strains from the Lipomyces genera. In the primary selection stage, a nitrogen-limited mineral medium (-NMM) that contained a mixture of glucose and xylose as a carbon source was used to evaluate the lipid-accumulating abilities of the yeast strains. The strains L. doorenjongii, L. orientalis, and L. starkeyi were selected as the potential strains in the primary selection. These three strains exhibited a remarkable ability to simultaneously assimilate glucose and xylose and achieved a cell biomass of more than 30â¯g/L. The values for lipid content in the selected strains were 57.89â¯Â±â¯1.92, 56.38â¯Â±â¯1.93, and 77.14â¯Â±â¯1.55% for L. doorenjongii, L. orientalis, and L. starkeyi, respectively. In the secondary selection, when the -NMM medium contained an inhibitory chemical compound (ICC), the selected strains showed a different tolerance level against each of the typical inhibitor compounds. However, L. starkeyi accumulated the highest lipid content and yield at 68.24â¯Â±â¯2.48% and 0.19â¯Â±â¯0.00 (w/w), respectively. L. starkeyi accumulated high levels of intracellular lipid and tolerated the ICC. The composition of fatty acid methyl esters (FAMEs) was unaltered by the presence of ICC and the major FAMEs consisted of oleic, palmitic, stearic, palmitoleic and linoleic acids.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Ario Betha Juanssilfero, Prihardi Kahar, Rezky Lastinov Amza, Nao Miyamoto, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Ahmad Thontowi, Yopi Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo,