Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6483993 | Biochemical Engineering Journal | 2016 | 7 Pages |
Abstract
Expansion of human mesenchymal stem cells (hMSCs) in bioreactor while preserving their innate properties is important in translation of hMSC-based therapy to clinical applications. The present study investigates the feasibility of hMSC expansion in a 2.5Â L CelliGen® 310 Bioreactor packed with Fibra-Cel® disks. After 9 days of expansion, a 9.2-fold increase in cell number with the population doubling (PD) time of 2.8 days (67.2Â h) was achieved and that the specific glucose consumption and lactate production were measured to be 12.48Â pmol/cell/day and 20.95Â pmol/cell/day, respectively. hMSCs harvested from the bioreactor maintained their properties based on the analysis of phenotypic surface markers, colony forming unit-fibroblasts (CFU-F) number, and multilineage differentiation ability. The results demonstrate the feasibility and the potential of the fibrous bed bioreactor for large scale hMSC expansion.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Ang-Chen Tsai, Yijun Liu, Teng Ma,