Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6484052 | Biochemical Engineering Journal | 2014 | 7 Pages |
Abstract
Extracellular lipase of the yeast Candida rugosa was produced via high cell density fed-batch fermentations using palm oil as the sole source of carbon and energy. Feeding strategies consisted of a pH-stat operation, foaming-dependent control and specific growth rate control in different experiments. Compared to foaming-dependent feeding and the pH-stat operation, the specific growth rate control of feeding proved to be the most successful. At the specific growth rate control set at 0.05 hâ1, the final lipase activity in the culture broth was the highest at â¼700 U Lâ1. This was 2.6-fold higher than the final enzyme activity obtained at a specific growth rate control set at 0.15 hâ1. The peak enzyme concentration achieved using the best foaming-dependent control of feeding was around 28% of the peak activity attained using the specific growth rate control of feeding at 0.05 hâ1. Similarly, the peak enzyme concentration attained using the pH-stat feeding operation was a mere 9% of the peak activity attained by specific growth rate control of feeding at a set-point of 0.05 hâ1. Fed-batch fermentations were performed in a 2 L stirred-tank bioreactor (30 °C, pH 7) with the dissolved oxygen level controlled at 30% of air saturation.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
M.N.I. Salehmin, M.S.M. Annuar, Y. Chisti,