| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6484060 | Biochemical Engineering Journal | 2014 | 10 Pages |
Abstract
- A scalable method based on agitation theory for harvesting hMSCs from microcarriers.
- Harvesting involves cell detachment from microcarriers followed by separation step.
- A combined short burst of intense agitation plus enzyme detaches cells in ~7Â min.
- After detachment, cells are not be damaged being smaller than Kolmogorov microscale.
- After harvest, cells retain all their usual quality attributes.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Alvin W. Nienow, Qasim A. Rafiq, Karen Coopman, Christopher J. Hewitt,