Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6484110 | Biochemical Engineering Journal | 2013 | 10 Pages |
Abstract
The effect of turbulence on suspended cells is one of the most complex problems in the scale-up of cell cultures. In the present paper, a direct comparison of the effects of turbulence on suspension cultures of Rubia tinctorum in a standard bioreactor and in shake flask cultures was done. A procedure derived from the well known global method proposed by Nishikawa et al. (1977) [39] was applied. Standard flasks and four-baffled shake flasks were used. The effect of turbulence and light irradiation on cell viability, biomass, and anthraquinones (AQs) production was evaluated. The biomass concentration and AQs production obtained using baffled shake flasks agitated at 360Â rpm were similar to that achieved in R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450Â rpm, previously published (Busto et al., 2008 [17]). The effect of light on AQs production was found to be very significant, and a difference of up to 48% was found in cells with and without illumination after 7 days of culture. It is concluded that this down-scaled and simple flask culture system is a suitable and valid small scale instrument for the study of intracellular mechanisms of turbulence-induced AQs production in R. tinctorum suspension cultures.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
VÃctor D. Busto, Ariel Calabró-López, Julián RodrÃguez-Talou, Ana M. Giulietti, José C. Merchuk,