Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6484421 | Biomaterials | 2018 | 28 Pages |
Abstract
In this paper, we present the development and systematic evaluation of the NICHE in vitro, and the in vivo validation with encapsulated testosterone-secreting Leydig cells in Rag1â/â castrated mice. Enhanced subcutaneous vascularization of NICHE via platelet-rich plasma (PRP) hydrogel coating and filling was demonstrated in vivo via a chorioallantoic membrane (CAM) assay as well as in mice. After establishment of a pre-vascularized bed within the NICHE, transcutaneously transplanted Leydig cells, maintained viability and robust testosterone secretion for the duration of the study. Immunohistochemical analysis revealed extensive Leydig cell colonization in the NICHE. Furthermore, transplanted cells achieved physiologic testosterone levels in castrated mice. The promising results provide a proof of concept for the NICHE as a viable platform technology for autologous cell transplantation for the treatment of a variety of diseases.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Marco Farina, Corrine Ying Xuan Chua, Andrea Ballerini, Usha Thekkedath, Jenolyn F. Alexander, Jessica R. Rhudy, Gianluca Torchio, Daniel Fraga, Ravi R. Pathak, Mariana Villanueva, Crystal S. Shin, Jean A. Niles, Raffaella Sesana, Danilo Demarchi,