Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6485085 | Biomaterials | 2016 | 10 Pages |
Abstract
Strategies to enhance survival and direct the differentiation of stem cells in vivo following transplantation in tissue repair site are critical to realizing the potential of stem cell-based therapies. Here we demonstrated an effective approach to promote neuronal differentiation and maturation of human fetal tissue-derived neural stem cells (hNSCs) in a brain lesion site of a rat traumatic brain injury model using biodegradable nanoparticle-mediated transfection method to deliver key transcriptional factor neurogenin-2 to hNSCs when transplanted with a tailored hyaluronic acid (HA) hydrogel, generating larger number of more mature neurons engrafted to the host brain tissue than non-transfected cells. The nanoparticle-mediated transcription activation method together with an HA hydrogel delivery matrix provides a translatable approach for stem cell-based regenerative therapy.
Related Topics
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Chemical Engineering
Bioengineering
Authors
Xiaowei Li, Stephany Y. Tzeng, Xiaoyan Liu, Markus Tammia, Yu-Hao Cheng, Andrew Rolfe, Dong Sun, Ning Zhang, Jordan J. Green, Xuejun Wen, Hai-Quan Mao,