Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6486701 | Biotechnology Advances | 2017 | 33 Pages |
Abstract
Microbial production of monoterpenes is often limited by their cytotoxicity and in vivo conversion. Therefore, alleviating cytotoxicity and reducing conversion by chassis engineering are highly desirable. On the other hand, engineering key enzymes is also critical for improving monoterpenes production through facilitating the biosynthesis process. Here we critically review recent advances in cytotoxicity alleviation, reducing in vivo conversion, selecting geranyl diphosphate synthase and engineering monoterpene synthases. These achievements would lead to the development of superior chassis with improved tolerance to cytotoxicity and rationally tailored metabolites profiles to improve titer, yield and productivity for the production of monoterpenes by microbial cells.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Lu Zhang, Wen-Hai Xiao, Ying Wang, Ming-Dong Yao, Guo-Zhen Jiang, Bo-Xuan Zeng, Ruo-Si Zhang, Ying-Jin Yuan,