| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6487600 | Current Opinion in Biotechnology | 2016 | 8 Pages |
Abstract
- The machinery from CRISPR-Cas immune systems can be engineered into powerful genome editing machines.
- The sgRNA:Cas9 technology is a convenient novel tool for efficient alteration of bacterial genomes.
- Self-targeting by CRISPR is an effective screening tool for selection of low-frequency genotypes.
- In addition to editing genomic sequences, engineered variants of Cas9 may be harnessed for transcriptional control, epigenetics, and chromosome imaging.
- This technology holds much promise for implementation in probiotics and starter cultures of industrial interest.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Rodolphe Barrangou, Jan-Peter van Pijkeren,