| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6487870 | Current Opinion in Biotechnology | 2014 | 7 Pages |
Abstract
The IgG1 has N-terminal glutamic acid (E) and glutamine (Q). Conversion of E to pyroglutamate (pE) results in no charge change while that of glutamine (Q) to pE results in a loss of amine group. C-terminal lysine is often cleaved off in the bioreactor culture. The terminal heterogeneities can be detected by many analytical methods. A simple CEX profile was shown.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Kurt Brorson, Audrey Y Jia,