| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6487883 | Current Opinion in Biotechnology | 2014 | 5 Pages |
Abstract
- Fab arm exchange is the swapping of Heavy-Light chain units between two IgG4.
- Hinge and CH3 domain residues determine IgG4 ability to engage in Fab arm exchange.
- S228P mutation in the hinge region blocks Fab-arm exchange in vitro and in vivo.
- S228P confers stability properties to IgG4 equivalent to those of IgG1.
- IgG4 Fab arm exchange principles can be used to produce bispecific IgG1 in vitro.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Xiaoyu Yang, Alexandre Ambrogelly,