| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6488276 | Enzyme and Microbial Technology | 2015 | 9 Pages |
Abstract
- Escherichia coli was engineered for the production of caffeic acid.
- Tyrosine ammonia lyase (TAL) converted 3Â mM of tyrosine to 2.62Â mM p-coumaric acid.
- TAL and 4-coumarate 3-hydroxylase (C3H) converted tyrosine in 1Â mM caffeic acid.
- This is the first study that shows caffeic acid production using TAL and CYP199A2.
- TAL and CYP199A2 converted tyrosine in 1.56Â mM caffeic acid.
Keywords
E. coliMCSS4-coumarate-CoA ligase4CLC3HPALC4HRBSp-Coumaric acidTyrosine ammonia lyaseTALSDS-PAGEBiosynthesisTyrosineribosome binding siteMultiple cloning sitesSynthetic biologyCinnamate-4-hydroxylasePhenylalanine ammonia lyasepolymerase chain reactionPCRCaffeic acidHPLChigh-performance liquid chromatography
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
J.L. Rodrigues, R.G. Araújo, K.L.J. Prather, L.D. Kluskens, L.R. Rodrigues,