Codon optimization, promoter and expression system selection that achieved high-level production of Yarrowia lipolytica lipase in Pichia pastoris

- The codons of Y. lipolytica lipase gene were optimized by de novo gene design and synthesis.
- Codon optimized lipase gene has significantly higher expression level than the native gene.
- PAOX1 controlled lipase gene has higher expression level than PFLD1 controlled.
- Lipase gene in Pichia GS115 has equal activity than newly developed PichiaPink system.
- Lipase activity of codon optimized recombinants in fermenter reached 38,500 U/mL.