Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6489568 | Journal of Bioscience and Bioengineering | 2018 | 7 Pages |
Abstract
AD-1, an aerobic denitrifier, was isolated from activated sludge and identified as Pseudomonas stutzeri. AD-1 completely removed NO3- or NO2- and removed 99.5% of NH4+ during individual culturing in a broth medium with an initial nitrogen concentration of approximately 50 mg Lâ1. Results showed that larger amounts of nitrogen were removed through assimilation by the bacteria. And when NH4+ was used as the sole nitrogen source in the culture medium, neither NO2- nor NO3- was detected, thus indicating that AD-1 may not be a heterotrophic nitrifier. Only trace amount of N2O was detected during the denitrification process. Single factor experiments indicated that the optimal culture conditions for AD-1 were: a carbon-nitrogen ratio (C/N) of 15, a temperature of 25°C and sodium succinate or glucose as a carbon source. In conclusion, due to the ability of AD-1 to utilize nitrogen of different forms with high efficiencies for its growth while producing only trace emissions of N2O, the bacterium had outstanding potential to use in the bioremediation of high-nitrogen-containing wastewaters. Meanwhile, it may also be a proper candidate for biotreatment of high concentration organic wastewater.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Hui Qing, Oscar Omondi Donde, Cuicui Tian, Chunbo Wang, Xingqiang Wu, Shanshan Feng, Yao Liu, Bangding Xiao,