Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6490047 | Journal of Bioscience and Bioengineering | 2018 | 7 Pages |
Abstract
Transgene expression is regulated through several steps, this study focuses on the mRNA translation step. The expression level of transgenes can be increased by 5â²-untranslated region (5â²UTR) sequences in certain genes which act as translational enhancers. On the other hand, translation in most mRNA species is repressed by growth, development, and stress events. There is a possibility that transgene mRNA is also repressed in these conditions, despite the use of a translational enhancer. Therefore, a consistently efficient translational enhancer is needed to develop a reliable transgene expression system. Herein we searched for mRNAs translated stably under different growth, development and environmental conditions using data sets of polysome fraction assays and microarray analysis. Correct 5â²UTR sequences of candidate genes were determined by cap analysis of gene expression and we tested translational ability of the candidate 5â²UTRs by reporter assays. We found the 5â²UTR of cold-regulated 47 gene to be an effective translational enhancer, contributing to stable high-level expression under various conditions.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Shotaro Yamasaki, Yuji Sanada, Ryoji Imase, Hideyuki Matsuura, Daishin Ueno, Taku Demura, Ko Kato,