Establishment of a five-enzyme cell-free cascade for the synthesis of uridine diphosphate N-acetylglucosamine

Article ID	Journal	Published Year	Pages	File Type
6490127	Journal of Biotechnology	2018	40 Pages	PDF

Keywords

uridine diphosphate-N-acetylglucosamine ADP UMP MCA UTP UDP IMAC GlcNAc OD600 IPTG UDP-GlcNAc RPM BWM GTP HCl FCC uridine diphosphate Adenosine Triphosphate ATP adenosine diphosphate SDS-PAGE Sodium dodecyl sulfate polyacrylamide gel electrophoresis Uridine triphosphate isopropyl β-D-1-thiogalactopyranoside His-tag Metabolic control analysis GDP Cell-free systems flux control coefficient Kinetic modeling N-acetylglucosamine hydrochloride GalU Inorganic pyrophosphatase immobilized metal affinity chromatography Guanosine triphosphate guanosine diphosphate

Related Topics

Physical Sciences and Engineering Chemical Engineering Bioengineering

Establishment of a five-enzyme cell-free cascade for the synthesis of uridine diphosphate N-acetylglucosamine

Authors

Reza Mahour, Jan Klapproth, Thomas F.T. Rexer, Anna Schildbach, Steffen Klamt, Markus Pietzsch, Erdmann Rapp, Udo Reichl,