Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6490816 | Journal of Biotechnology | 2015 | 6 Pages |
Abstract
A halohydrin dehalogenase (HHDH-PL) from Parvibaculum lavamentivorans DS-1 was characterized and applied to determine azide and cyanide in the water. In this methodology, HHDH-PL catalysed azide and cyanide to react with butylene oxide and form corresponding β-substituted alcohols 1-azidobutan-2-ol (ABO) and 3-hydroxypentanenitrile (HPN) that could be quantitatively detected by gas chromatograph. The detection calibration curves for azide (R2 = 0.997) and cyanide (R2 = 0.995) were linear and the lower limits of detection for azide and cyanide were 0.1 and 0.3 mM, respectively. Several other nucleophiles were identified having no effect on the analysis of azide and cyanide, excepting nitrite which influenced the detection of cyanide. This was the first report of a biological method to determine the inorganic azide and cyanide by converting them to the measurable organics.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Nan-Wei Wan, Zhi-Qiang Liu, Feng Xue, Yu-Guo Zheng,