Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6490895 | Journal of Biotechnology | 2015 | 35 Pages |
Abstract
Results show that expanding cells in a continuous perfusion operation mode provided a higher expansion ratio, and led to a shift in cells' metabolism. TFF operated either in continuous or discontinuous allowed to concentrate cells, with high cell recovery (>80%) and viability (>95%); furthermore, continuous TFF permitted to operate longer with higher cell concentrations. Continuous diafiltration led to higher protein clearance (98%) with lower cell death, when comparing to discontinuous diafiltration. Overall, an integrated process allowed for a shorter process time, recovering 70% of viable hMSC (>95%), with no changes in terms of morphology, immunophenotype, proliferation capacity and multipotent differentiation potential.
Related Topics
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Authors
Bárbara Cunha, Tiago Aguiar, Marta M. Silva, Ricardo J.S. Silva, Marcos F.Q. Sousa, Earl Pineda, Cristina Peixoto, Manuel J.T. Carrondo, Margarida Serra, Paula M. Alves,