Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6491007 | Journal of Biotechnology | 2015 | 7 Pages |
Abstract
Hydrostatic pressure and perfusion have been shown to regulate the chondrogenic potential of articular chondrocytes. In order to compare the effects of hydrostatic pressure plus perfusion (HPP) and perfusion (P) we investigated the complete gene expression profiles of human chondrocytes under HPP and P. A simplified bioreactor was constructed to apply loading (0.1Â MPa for 2Â h) and perfusion (2Â ml) through the same piping by pressurizing the medium directly. High-density monolayer cultures of human chondrocytes were exposed to HPP or P for 4 days. Controls (C) were maintained in static cultures. Gene expression was evaluated by sequencing (RNAseq) and quantitative real-time PCR analysis. Both treatments changed gene expression levels of human chondrocytes significantly. Specifically, HPP and P increased COL2A1 expression and decreased COL1A1 and MMP-13 expression. Despite of these similarities, RNAseq revealed a list of cartilage genes including ACAN, ITGA10 and TNC, which were differentially expressed by HPP and P. Of these candidates, adhesion related molecules were found to be upregulated in HPP. Both HPP and P treatment had beneficial effects on chondrocyte differentiation and decreased catabolic enzyme expression. The study provides new insight into how hydrostatic pressure and perfusion enhance cartilage differentiation and inhibit catabolic effects.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Ge Zhu, Susanne Mayer-Wagner, Christian Schröder, Matthias Woiczinski, Helmut Blum, Ilaria Lavagi, Stefan Krebs, Julia I. Redeker, Andreas Hölzer, Volkmar Jansson, Oliver Betz, Peter E. Müller,