Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6491068 | Journal of Biotechnology | 2015 | 9 Pages |
Abstract
- Cationic microparticles extract recombinant proteins upon contact with E. coli cells.
- Endogenous component concentration in homogenate is three orders of magnitude lower.
- E. coli cells form flocs upon contact with microparticles facilitating separation.
- Selective desorption of bound protein by NaCl without destroying the floc network.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Alexandru Trefilov, Moritz Imendoerffer, Gerhard Sekot, Florian Strobl, Alois Jungbauer, Rainer Hahn,