Immunosensor for trace penicillin G detection in milk based on supported bilayer lipid membrane modified with gold nanoparticles

In this work, we developed an immunosensor for electrochemical detection of penicillin G at trace level. The biosensor was fabricated by immobilizing anti-penicillin G in a supported bilayer lipid membrane (s-BLM) modified with gold nanoparticles, and the modified electrodes were characterized by the scanning electron microscope (SEM), cyclic voltammetry and electrochemical impedance spectroscopy. The biosensor was able to detect penicillin G with a linear correlation ranging from 3.34 × 10−3 ng/L to 3.34 × 103 ng/L and a detection limit of 2.7 × 10−4 ng/L, much lower than the maximum residue limit (MRL) of penicillin G in milk (4 ppb, equal to 4 × 103 ng/L) set out by the European Union. The mean coefficient variation (CV) of the intra-assays and the inter-assays were 5.4% and 7.7%, respectively. In addition, the concentration of penicillin G in milk samples determined by this biosensor was in good agreement with that determined by high performance liquid chromatography (HPLC) assay.