Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6491205 | Journal of Biotechnology | 2015 | 7 Pages |
Abstract
- Protein engineering was performed on thermo-stable Alcaligenes faecalis penicillin G acylase.
- A screened mutant showed up to 800-fold increased catalytic efficiency for activated acyl donor.
- 95% of 6-APA could be converted to ampicillin in aqueous medium by mutant PGA.
- Process productivity of enzymatic synthesis of ampicillin was improved by more than 130 times.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Senwen Deng, Erzheng Su, Xiaoqiang Ma, Shengli Yang, Dongzhi Wei,