Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6491467 | Journal of Biotechnology | 2014 | 9 Pages |
Abstract
In the current study, different protein expression profiles in a strain Brevundimonas sp. ZF12, isolated from the aqueous zone containing high levels of radiation, were characterized following exposure to cadmium (II) using a proteomic strategy. In order to gain a deeper understanding of the cellular events that allow this strain to survive and undergo cadmium adaptation and sorption, the strain was tested under three experimental conditions of 5, 10 and 30Â ppm cadmium (II) ions stress. Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry were used to identify the differentially expressed proteins under cadmium (II) stress. 20 differentially expressed spots were successfully identified by MS/MS analysis. These proteins are involved in DNA repair and protection, amino acid metabolism, nucleotide metabolism, energy homeostasis, oxidative stress response, redox homeostasis, protein folding and heat-shock response. The results obviously indicate that the ZF12 strain tends to endure the cadmium (II) stress conditions by modification in many aspects of its cellular physiology and metabolism.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Nasrin Masoudzadeh, Leila Alidoust, Nima Samie, Hamidreza Hajfarajollah, Hakimeh Sharafi, Sima Modiri, Hossein Shahbani Zahiri, Hojatollah Vali, Kambiz Akbari Noghabi,