Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6491480 | Journal of Biotechnology | 2014 | 7 Pages |
Abstract
- l-Phenylglycine (l-Phg) was firstly produced from glucose by fermentation.
- Three enzymes from actinomyces were expressed in E. coli and purified.
- New cycle multi-enzyme cascade reactions were developed for l-Phg production.
- Optimizing gene expression and transamination increased l-Phg yield by 224-fold.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Shuang Ping Liu, Rui Xia Liu, Ashraf A.M.M. El-Rotail, Zhong Yang Ding, Zheng Hua Gu, Liang Zhang, Gui Yang Shi,