| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6491624 | Journal of Biotechnology | 2014 | 10 Pages |
Abstract
- Optimal conditions for preparation of high density thiol functionalized cryogels.
- Thiol-functionalized cryogel as reducing solid phase for reduction of GSSG.
- Cryogel-mediated selective reduction of cysteine in recombinant human growth hormone.
- Potentially scalable setup identified.
- Key optimization parameters identified.
Keywords
DTNBdimethylacrylamidePEG-MALDMAATEMEDGSSGPEG-DAGSHDTTHEPES5,5′-dithiobis-(2-nitrobenzoic acid)APsN,N,N′,N′-tetramethylethylenediaminetris(2-carboxyethyl)phosphineEDTAethylenediaminetetracetic acidtriethanolaminedithiothreitolTCEPSEMScanning electron microscopyHuman growth hormoneammonium persulfateTEAreduced glutathioneglutathione disulfideMacroporous monolith
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Gry Ravn Jespersen, Finn Matthiesen, Anja Kallesøe Pedersen, Henrik Sune Andersen, Harald Kirsebom, Anders Lærke Nielsen,