| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6491657 | Journal of Biotechnology | 2013 | 9 Pages |
Abstract
- Co-expression of human O-GlcNAc transferase and substrate proteins in Escherichia coli.
- In vivo O-GlcNAc-modification of human CREB1 and ABL2 in E. coli.
- β-N-acetylglucosaminidase inhibition increases O-GlcNAc levels.
- E. coli NagZ deglycosylates O-GlcNAc-modified proteins in vivo.
- NMR assay shows NagZ deglycosylates O-GlcNAc-modified peptide in vitro.
Keywords
E. coliO-GlcNAcaseOGAO-linked β-N-acetylglucosamineUDP-GlcNAcAbl2O-GlcNAc transferaseTRISO-GlcNAcMurNAcCREB1CXCOGTD2ODSSPUGNAcNaClLOQnuclear magnetic resonance4,4-dimethyl-4-silapentane-1-sulfonic acidN-acetylmuramic acidS/Nβ-N-acetylglucosaminidaseDeuterated waterSDS-PAGETris(hydroxymethyl)aminomethaneNMRMichaelis–Menten constantSodium chlorideLuria brothlimit of quantitationSignal-to-noise ratioCation exchange chromatographyGlycosidase
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Octavia Y. Goodwin, Maggie S. Thomasson, Aaron J. Lin, Michelle M. Sweeney, Megan A. Macnaughtan,