| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6491743 | Journal of Biotechnology | 2013 | 36 Pages |
Abstract
A stable CHO cell line producing >200Â mg/L of rhLF was developed and established. rhLF was purified by a single-step cation-exchange chromatography procedure. The highly homogenous rhLF has a molecular weight of approximately 80Â kDa. MALDI-TOF mass spectrometric analysis revealed N-linked, partially sialylated glycans at two glycosylation sites, typical for human milk LF. This novel rhLF showed a protective effect against oxidative stress in a similar manner to its natural counterpart. In addition, rhLF revealed a modulatory effect on cellular redox via upregulation of key antioxidant enzymes. These data imply that the CHO-derived rhLF is fully compatible with the native molecule, thus it has promise for human therapeutic applications.
Keywords
PrdxhLFCFULPSCOXMTXPTGSBCGMALDI-TOFGPXTransferrinHEKROSAntioxidantcyclooxygenaseinflammationChoGene expressionEnzyme-linked immunosorbent assayELISASODChinese hamster ovary cellsSuperoxide dismutaseICP-MSinductively coupled plasma mass spectrometryRecombinant human lactoferrinlipopolysaccharideMethotrexatematrix-assisted laser desorption/ionization time of flightcolony forming unitsProstaglandin-endoperoxide synthasePeroxiredoxinsglutathione peroxidaseReactive oxygen species
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Marian L. Kruzel, Jeffrey K. Actor, MichaÅ Zimecki, Jasen Wise, Paulina PÅoszaj, Shaper Mirza, Mark Kruzel, Shen-An Hwang, Xueqing Ba, Istvan Boldogh,