| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6491768 | Journal of Biotechnology | 2013 | 8 Pages |
Abstract
Geraniol is an important industrial material and a potential candidate of advanced biofuels. One challenge of microbial geraniol production is the toxicity to hosts. However, the poor understanding on geraniol tolerance mechanism is an obstacle for developing geraniol tolerant host. This study genome-widely screened a shot-gun DNA library of Escherichia coli and found that recA is able to confer geraniol tolerance in E. coli. The recA knockout mutant was found extremely sensitive to geraniol. Based on our data, it was deciphered that recA provided tolerance through SOS response network responding to DNA damage caused by geraniol. RecA-mediated SOS response activates the homologous recombinational repair by RecB and RecN for corrective DNA maintenance. This protection mechanism suggests an effective strategy to combat geraniol toxicity in E. coli.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Asad Ali Shah, Chonglong Wang, Sang-Hwal Yoon, Jae-Yean Kim, Eui-Sung Choi, Seon-Won Kim,