Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6491910 | Journal of Biotechnology | 2013 | 11 Pages |
Abstract
⺠Two Pseudomonas aeruginosa cosmids containing gene clusters for nitrate or nitrite respiration were isolated. ⺠Transfer of these cosmids in the obligate aerobic Pseudomonas putida KT2440 generated two strains, which anaerobically reduced nitrate and nitrite, respectively. ⺠Microarray analysis confirmed expression of all structural, maturation and regulatory P. aeruginosa denitrification genes. ⺠Recombinantly produced P. aeruginosa enzyme systems allowed for anaerobic life of P. putida KT2440. ⺠Expression of P. putida genes encoding a putative copper stress response operon was observed.
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Authors
Annika Steen, F. Ãzde Ãtkür, José Manuel Borrero-de Acuña, Boyke Bunk, Louisa Roselius, Bruno Bühler, Dieter Jahn, Max Schobert,