Article ID Journal Published Year Pages File Type
6491937 Journal of Biotechnology 2013 8 Pages PDF
Abstract
► Tripartite fusion proteins of heparinase I were constructed and compared. ► Effects of fusion order and linker sequence on characteristics of fusion proteins were studied. ► Linker sequences have significant effects on protein expression, heparinase I activity and thermostability. ► Optimization of linker combination at different domain junctions were studied.
Related Topics
Physical Sciences and Engineering Chemical Engineering Bioengineering
Authors
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