| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6491944 | Journal of Biotechnology | 2013 | 7 Pages |
Abstract
⺠A new set of thermo-responsive ELP-z/zz domain fusions was constructed as the capturing scaffolds for the direct purification and recovery of antibodies. ⺠The ELP-zz scaffolds was used in four repeated binding and elution cycles with no observable decrease in the purification performance. ⺠The same binding and elution cycle was successfully implemented for the purification of monoclonal antibodies from hybridoma culture supernatant with close to 100% recovery.
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Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Bhawna Madan, Garima Chaudhary, Steven M. Cramer, Wilfred Chen,