| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6492058 | Journal of Biotechnology | 2012 | 7 Pages |
Abstract
⺠We immobilize Streptag II-TEV protease in oriented manner on streptavidin matrix. ⺠Affinity immobilized enzyme retains more than 80% of its activity. ⺠Streptag II/streptavidin based immobilization improves 2-4 times enzyme stability. ⺠Affinity immobilization offers reuse of TEV protease during long periods. ⺠TEV proteolytic column shortens cleavage process of streptagged fusion proteins.
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Authors
Baligh Miladi, Ahmed El Marjou, Guilhem Boeuf, Hassib Bouallagui, Florence Dufour, Patrick Di Martino, Abdellatif Elm'selmi,